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hank's balanced saline solution (hbss (Thermo Fisher)

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Thermo Fisher hank's balanced saline solution (hbss
Hank's Balanced Saline Solution (Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solution/nct06063551-30-4-11
Average 90 stars, based on 1 article reviews

hank's balanced saline solution (hbss - by Bioz Stars, 2026-09

90/100 stars

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Blocking Assay:

Article Title: TROP2/claudin program mediates immune exclusion to impede checkpoint blockade in breast cancer.
Article Snippet: .. After washing with PBS for three times every 5 min to remove OCT and then incubate in blocking solution (5% normal donkey serum, 0.25% Triton X- 100 in PBS) for 1 hour at room temperature, followed by incubation with anti- claudin 7 antibody (1:100 dilution; Invitrogen, 34–9100) and anti- Trop2 antibody (10 μg/μl; RD system, AF1122) in blocking solution overnight at 4°C. ..

Incubation:

Dispersion:

Article Title: Controlling PEDOT:PSS electropolymerization for label-free optical recording of bioelectric potentials.
Article Snippet: For spin-coated PEDOT:PSS films, a dispersion consisting of 16 mL PEDOT:PSS (Clevios PH1000, Heraeus), 4 mL ethylene glycol (Sigma-Aldrich), 24 mL sodium dodecylbenzenesulfonate (Tokyo Chemical Industry Co., Ltd), and 200 mL (3-glycidyloxypropyl)trimethoxysilane (Sigma-Aldrich) was prepared by vortex mixing followed by sonication for 5 min and filtration through a 1.2 mm cellulose acetate syringe filter (add vendor here). .. The dispersion was spin-coated onto ITO substrates at 5500 rpm using a static dispense method and annealed at 140 1C for at least 1 h. Prior to measurements, all spin-coated films were electrochemically pre-conditioned by cycling the potential between 0.3 V and 0.3 V at a scan rate of 100 mV s 1 for 5 cycles in HEPES (4-(2-hydroxyethyl) 1-piperazineethanesulfonic acid)-buffered Tyrode’s solution (1 mL 1 M CaCl2, 500 mL 1 M MgCl2, 5 mL HEPES stock (1 M) (Gibco, Thermo Fisher), 4.237 g NaCl (145 mM), 149 mg KCl (4 mM), 500 mL Milli-Q water. ..

other:

Article Title: An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections
Article Snippet: Next, to prepare the samples for hybridization, both cells and tissue samples were rinsed once then incubated overnight in a buffer containing 50% formamide (ThermoFisher) / 2x SSC / 50 mM sodium phosphate buffer, at room temperature in the dark.

Immunofluorescence:

Article Title: Analysis of cortical dysplasias using b-tensor encoding diffusion MRI in an animal model.
Article Snippet: Immunofluorescence was performed using the primary antibodies anti-Myelin Basic Protein (MBP; 1:500; abcam), anti-Neuronal Nuclear Protein (NeuN; 1:350; abcam), and anti-Glial Fibrillary Acidic Protein (GFAP; 1:350; Sigma-Aldrich). .. For the triple immunofluorescence staining, the tissue sections were blocked with Bovine Serum Albumin (BSA; Sigma-Aldrich) 2% solution + 0.3% triton X-100 (ThermoFisher) in 1X PBS for 45 min. ..

Staining:

Sterility:

Article Title: The adaptor protein TASL is required for age-related B cell emergence and lupus-like disease development in mice.
Article Snippet: .. For SRBC immunization, 1 ml of sterile SRBCs in Alsever’s solution (Thermo Fisher Scientific) was washed twice with 10 ml ice-cold PBS, then reconstituted in 2–3 ml of PBS. ..